rabbit anti human f3 antibody  (Danaher Inc)

Journal: Cell

Article Title: Spatiotemporal analysis of human intestinal development at single-cell resolution

doi: 10.1016/j.cell.2020.12.016

Figure Lengend Snippet: Intestinal morphogen gradients in specific cell types and spatial locations (A) Graph visualization of morphogen molecule STRING interactome. Communities enriched for EGFR, FGF, Hedgehog, HIPPO, NOTCH, RTK, TGF-beta and WNT signaling pathways are highlighted in dashed ellipses. Nodes are colored by scRNA-Seq module. Nodes unassigned to a module are shown in grey (NA). (B) Individual morphogen module overview shown as a module score overlay in ST spots in slides at 19 and 12 PCW colon and a dotplot showing module gene expression at compartment level for ST module 3 which captures endothelial, pericyte and fibroblast morphogens (i) and scRNA-Seq module 8, which captures epithelial morphogens (ii). All spot overlays shown are plotted over ST H&E sections A4 (top) and A3 (bottom, rotated). Full images are available in . (C) (i) Violin plot showing expression of RSPO3 over developmental time course in scRNA-Seq of muscularis compartment cells. (ii) Dotplot showing the expression of mesothelium and muscularis-specific morphogen scRNA-Seq module 5 genes (D) Individual morphogen module overview shown as module score overlay in ST spots in slides at 19 and 12 PCW colon and a doplot showing expression of fibroblast-specific morphogen spatial module 6. All spot overlays are plotted over ST H&E sections A4 (top) and A3 (bottom, rotated). Full images are available in . (E) Violin plots depicting selected S2 genes ( POSTN (i) and BMP3 (ii)) that show locational and time-course differences in expression. (F) Representative images of colonic sections from 11, 15 and 20 PCW embryonic tissue stained for F3 protein by IHC. Brown arrows within the 11 PCW sample indicate positive F3 staining below newly developing invaginations/hillocks in epithelium ( n = 3 for each individual image with +-1pcw samples, 10x/20x magnification scale bar=360/180 μm)

Article Snippet: F3 rabbit anti human antibody , Sigma , Cat# HPA049292, RRID: AB_2680701.

Techniques: Expressing, Staining

Journal: Cell

Article Title: Spatiotemporal analysis of human intestinal development at single-cell resolution

doi: 10.1016/j.cell.2020.12.016

Figure Lengend Snippet:

Article Snippet: F3 rabbit anti human antibody , Sigma , Cat# HPA049292, RRID: AB_2680701.

Techniques: Conjugation Assay, Recombinant, Modification, Plasmid Preparation, Expressing, Generated, Software

Journal: Kidney international

Article Title: Oxidized LDL stimulates the expression of TGF-beta and fibronectin in human glomerular epithelial cells.

doi: 10.1038/ki.1997.18

Figure Lengend Snippet: Fig. 4. Immunoperoxidase staining of human GECfor TGF-f3 (A, B) and fibronectin (A', B'). A and A' are the cells not exposed to ox-LDL. B and B' are the cells exposed to ox-LDL (50 rgIml) for 24 hours. TGF-13 (B) and fibronectin (B') were obviously increased in GEC exposed to ox-LDL. Comparable results were obtained in three sets of experiments.

Article Snippet: In separate experiments, GEC were incubated with ox-LDL in the presence of rabbit anti-human TGF-f3 antibody or nonspecific rabbit IgG (R&D Systems, Minneapolis, MN, USA) for 6 and 24 hours.

Techniques: Immunoperoxidase Staining